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KMID : 0525720150200040245
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Journal of Chitin and Chitosan
2015 Volume.20 No. 4 p.245 ~ p.250
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Chitosan-phytochemical Conjugates: Preparation, Antioxidant, and NO Inhibition in LPS-Stimulated Macrophages
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Je Jae-Young
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Abstract
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Chitosan-phytochemical conjugates were prepared by free radical-mediated conjugation method. Successful preparation of chitosan-p-coumaric acid, chitosan-quercetin, and chitosan-syringic acid was confirmed using 1 H NMR spectroscopy and the contents of each p-coumaric acid, quercetin, and syringic acid in the conjugates were also determined. Three conjugates were subjected to evaluate antioxidant activity and inhibition activity for nitric oxide (NO) production in lipopolysaccharide-stimulated RAW264.7 macrophages. Chitosan-quercetin and chitosan-syringic acid exhibited significantly (p<0.05) enhanced antioxidant activities compared to plain chitosan, which is prepared without addition of phytochemicals, but chitosan-p-coumaric acid showed similar antioxidant activity than those of plain chitosan. In lipid peroxidation assay, chitosan-quercetin only showed significantly (p<0.05) enhanced lipid peroxidation inhibition activity. In inhibition activity for NO production, chitosan-quercetin and chitosan-syringic acid significantly (p<0.05) inhibited NO production in LPS-stimulated RAW264.7 macrophages.
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KEYWORD
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Chitosan, Phytochemical, Antioxidant, NO production, Conjugation
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